Composite

Part:BBa_K313036:Design

Designed by: Tomoka Ao, Ryosuke Kamei, Ryo Kariyazono, Masahiko Shimizu, Kosuke Uekusa, Mami Nakatake   Group: iGEM10_UT-Tokyo   (2010-10-26)


MS2 phage cDNA (loading sequence and replicase-deficient)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal BamHI site found at 2057
    Illegal XhoI site found at 1048
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2437
    Illegal NgoMIV site found at 3309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1665
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 2257


Design Notes

We removed XbaI site, loading sequence and replicase region.


Source

We bought MS2 virus as RNA and made cDNA by reverse transcription.

References